Development of a new screening tool for cyber pornography. Psychometric properties of the Cyber Pornography Addiction Test (CYPAT)

Objective: Internet pornography addiction typically involves viewing, downloading and trading online pornography or engagement in adult fantasy role-play. There are some well-validated inventories measuring perceived addiction to internet pornography but these instruments are often too long for a functionally use and fast scoring. The aim of this study was to evaluate the psychometric properties of the cyber pornography addiction test (CYPAT), a new, brief, screening measure for assessing cyber pornography.Method: Participants of this study completed the CYPAT, the CPUI, the TAS-20 and the FACES-IV. Descriptive statistics were calculated and Exploratory Factor Analysis (EFA) and Confirmatory Factor Analysis (CFA) were applied.Results: Cronbach's alpha coefficient suggested excellent reliability of the measure. Results of this study revealed also good construct, convergent and divergent validity.Conclusions: CYPAT is a brief self-report screening scale composed of 11 items scored on a five-point Likert scale with good psychometrics properties. The implications of these findings for future theoretical and empirical research in this field are discusse

Pioneering Preclinical Research in Diffuse Intrinsic Pontine Glioma: Towards New Treatment Strategies

Kaspers, G.J.L. [Promotor]Vandertop, W.P. [Promotor]Noske, D.P. [Copromotor]Würdinger, T. [Copromotor

Lights and Shadows in the Use of Mesenchymal Stem Cells in Lung Inflammation, a Poorly Investigated Topic in Cystic Fibrosis

Mesenchymal stem cells (MSCs) are multipotent non-hematopoietic stem cells residing in many tissues, including the lung. MSCs have long been regarded as a promising tool for cell-based therapy because of their ability to replace damaged tissue by differentiating into the resident cell and repopulating the injured area. Their ability to release soluble factors and extracellular vesicles has emerged as crucial in the resolution of inflammation and injury. There is a growing literature on the use of MSCs and MSC secretome to hamper inflammation in different lung pathologies, including: asthma, pneumonia, acute lung injury (ALI), pulmonary hypertension, and chronic obstructive pulmonary disease (COPD). However, their potential therapeutic role in the context of Cystic Fibrosis (CF) lung inflammation is still not fully characterized. CF morbidity and mortality are mainly due to progressive lung dysfunction. Lung inflammation is a chronic and unresolved condition that triggers progressive tissue damage. Thus, it becomes even more important to develop innovative immunomodulatory therapies aside from classic anti-inflammatory agents. Here, we address the main features of CF and the implications in lung inflammation. We then review how MSCs and MSC secretome participate in attenuating inflammation in pulmonary pathologies, emphasizing the significant potential of MSCs as new therapeutic approach in CF

Metabolic control of stemness and differentiation

Prove crescenti evidenziano un ruolo fondamentale per il metabolismo nella fisiologia delle cellule staminali e nella specifica del lignaggio [1, 2]. Il metabolismo, infatti, non è più considerato solo una fonte di energia né un endpoint della regolazione genica. Invece, i metaboliti e l'ambiente nutritivo sono attori attivi nel determinare la segnalazione intracellulare e le attività enzimatiche e di conseguenza modulatori del destino delle cellule staminali. Inoltre, gli intermedi metabolici del metabolismo cellulare regolano i meccanismi epigenetici, comprese le modificazioni degli istoni, la metilazione del DNA e gli RNA non codificanti, modulando in tal modo il paesaggio e lo staminali dell'epigenoma globale [3]. Questo numero speciale riunisce 9 documenti per evidenziare i recenti sviluppi nel campo.Increasing evidence highlights a pivotal role for metabolism in stem cell physiology and lineage specification [1, 2]. Metabolism, indeed, is no longer considered merely an energy source nor an endpoint of gene regulation. Instead, metabolites and the nutrient environment are active players in determining intracellular signaling and enzymatic activities and consequently modulators of stem cell fate. Moreover, metabolic intermediates of cellular metabolism regulate epigenetic mechanisms, including histone modifications, DNA methylation, and noncoding RNAs, thereby modulating the global epigenome landscape and stemness [3]. This special issue brings together 9 papers to highlight recent developments in the field

Interactions between p300 and multiple NF-Y trimers govern cyclin B2 promoter function

The CCAAT box is one of the most common elements in eukaryotic promoters and is activated by NF-Y, a conserved trimeric transcription factor with histone-like subunits. Usually one CCAAT element is present in promoters at positions between -60 and -100, but an emerging class of promoters harbor multiple NF-Y sites. In the triple CCAAT-containing cyclin B2 cell-cycle promoter, all CCAAT boxes, independently from their NF-Y affinities, are important for function. We investigated the relationships between NF-Y and p300. Chromatin immunoprecipitation analysis found that NF-Y and p300 are bound to the cyclin B2 promoter in vivo and that their binding is regulated during the cell cycle, positively correlating with promoter function. Cotransfection experiments determined that the coactivator acts on all CCAAT boxes and requires a precise spacing between the three elements. We established the order of in vitro binding of the three NF-Y complexes and find decreasing affinities from the most distal Y1 to the proximal Y3 site. Binding of two or three NF-Y trimers with or without p300 is not cooperative, but association with the Y1 and Y2 sites is extremely stable. p300 favors the binding of NF-Y to the weak Y3 proximal site, provided that a correct distance between the three CCAAT is respected. Our data indicate that the precise spacing of multiple CCAAT boxes is crucial for coactivator function. Transient association to a weak site might be a point of regulation during the cell cycle and a general theme of multiple CCAAT box promoters

Inhibition of Bromodomain and Extraterminal Domain (BET) Proteins by JQ1 Unravels a Novel Epigenetic Modulation to Control Lipid Homeostasis

The homeostatic control of lipid metabolism is essential for many fundamental physiological processes. A deep understanding of its regulatory mechanisms is pivotal to unravel prospective physiopathological factors and to identify novel molecular targets that could be employed to design promising therapies in the management of lipid disorders. Here, we investigated the role of bromodomain and extraterminal domain (BET) proteins in the regulation of lipid metabolism. To reach this aim, we used a loss-of-function approach by treating HepG2 cells with JQ1, a powerful and selective BET inhibitor. The main results demonstrated that BET inhibition by JQ1 efficiently decreases intracellular lipid content, determining a significant modulation of proteins involved in lipid biosynthesis, uptake and intracellular trafficking. Importantly, the capability of BET inhibition to slow down cell proliferation is dependent on the modulation of cholesterol metabolism. Taken together, these data highlight a novel epigenetic mechanism involved in the regulation of lipid homeostasis